Estimating the inhibitory effect of Lactobacillus isolated from vagina against some pathogens of genital infections in group of women.

Abstract

Thirty- five vaginal swab samples were obtained from women vagina.All samples
were subjected to conventional morphological and cultural characteristics, isolates
distributed between Lactobacillus acidophillus(23)and Lactobacillus fermentum (12).
Antibacterial activities were done by well diffusion and blank disk method. The
Lactobacillus was used as aprobiotic treatment against bacteriaisolates from vagina
.The isolated bacteria had strong activity against indicator strains.The results showed
that Lactobacillus which was isolated from vagina by well diffusion method was
effective against pathogenic isolates more than the Lactobacillus isolated by blank
disc method , the highs inhibitory effect of Lactobacillus isolates by well give an
inhibition zone reached to(18)mm , while the Lactobacillus by disc was lower with
inhibition zone (12-15) mm. Also the results revealed that Lactobacillus spp. whole
cell of Lactobacillus was more effective against pathogenic isolates more than
usedsupernatant . The supernatant did not show any activity when was treated with
NaOH and adjusted to pH 7. This indicates that the organic acid produced by the
Lactobacillus isolates was may be actually responsible for the inhibition of the
indicator bacteria.The result show that theLactobacillus strains could be considered
asapotential antimicrobial probiotic strains against some human vaginal pathogens
and should be further studied for their human health benefits.