Evaluation of Antioxidant Activity of Acoholic Extracts from Pleurotus ostreatus (P2) In Vivo

Abstract

In this study found treatment mice exposed to CCl4 with the alcoholic extract of Pleurotus ostreatus (P2) at (25) mg/ml appeared to exert a beneficial effect since the activities of catalase, was significantly (p<0.05) higher in liver treatment with alcoholic extract of P. ostreatus (P2) (37.290+2.638) μmol/mg than in aqueous extract for the same fungus (36.557+2.231) μmol/mg. Super oxide dismutase (SOD) was significantly (p<0.05) higher in liver treatment with alcoholic extract of P. ostreatus (P2) at (25) mg/ml (17.863+0.454)U/mg than in aqueous extract (17.863+0.454) U/mg for the same fungus and same concentration. Glutathione peroxidase (Gpx) was significantly (p<0.05) higher in liver treatment with alcoholic extract of P. ostreatus(P2) at (25) mg/ml (29.260+1.666)μmol/mg than in aqueous extract (24.660+1.130)μmol/mg for the same fungus and same concentration. Alcoholic extract of P .ostreatus (P2) at (25) mg/ml induce a non-apoptotic, apoptosis was (0.0657+0.0047%) ,in aqueous extract was (0.0770+0.0060%). Histopathological study appeared liver cells exposed to CCl4, CCl4 made severe degeneration and necrotic change in liver parenchyma by evidence of apoptosis in some hepatocytes , in sinusoids showed slight congestion with blood vessel and thrombus formation. Liver cells of mouse exposed to CCl4 and treated with alcoholic mushroom extract and appeared the liver cells returned like control. the tissue treatment by aqueous extract of P.ostreatus (P2) at (25) mg/ml appeared mononuclear cells aggregation.