Assessment of pelA-carried Pseudomonas aeruginosa isolates in respect to biofilm formation

Mahmood Abd AL- Razzaq Hassan AL-Sheikhly; Laith N. Musleh; Harith J. F. Al-Mathkhury

doi:10.24996/ijs.2019.60.6.1

Authors

Mahmood Abd AL- Razzaq Hassan AL-Sheikhly Laboratory Department, Directorate of technical matters, Ministry of Health, Baghdad, Iraq
Laith N. Musleh Department of Biology, College of Science, University of AL-Anbar, AL-Anbar, Iraq
Harith J. F. Al-Mathkhury Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq

DOI:

https://doi.org/10.24996/ijs.2019.60.6.1

Keywords:

Biofilm, pelA gene, antibiotic resistance and PCR

Abstract

Owing to high antibacterial resistance of Pseudomonas aeruginosa, it could be considered as the main reason behind the nosocomial infections. P. aeruginosa has a well-known biofilm forming ability. The expression of polysaccharide encoding locus (pelA gene) by P. aeruginosa is essential for this ability. The purpose of the current research was to determine the biofilm formation in P. aeruginosa isolated from clinical samples and to evaluate the role of the selected PelA gene in biofilm formation using PCR method in Iraqi patients. Results revealed that 24 (96%) isolates were found to have the ability to form biofilm that was remarkably related to gentamicin resistance. Moreover, the pelA gene was found in all biofilm-producers. In conclusion, the results of the current study revealed that the P. aeruginosa biofilm-producer isolates were resistant to the antibiotics in question. Likewise, because of wide spreading, it appears that the pelA gene is related to biofilm formation.