Effectiveness of Eucalyptus camaldulensis Leaves Oil in Upregulating exoU expression in Pseudomonas aeruginosa

Nihad T. M.  Jaddoa; Mahmood A. H.  AL-Sheikhly; Israa A.M.  Aldobaissi; Harith J. F.  Al-Mathkhury

doi:10.24996/ijs.2024.65.10.%g

Authors

Nihad T. M. Jaddoa Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq, 10071 https://orcid.org/0000-0003-0102-033X
Mahmood A. H. AL-Sheikhly Laboratory Department, Directorate of Technical matters, Ministry of Health, Baghdad, Iraq
Israa A.M. Aldobaissi Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq, 10071 https://orcid.org/0000-0002-6729-1119
Harith J. F. Al-Mathkhury Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq, 10071 https://orcid.org/0000-0002-5414-9834

DOI:

https://doi.org/10.24996/ijs.2024.65.10.%25g

Keywords:

Eucalyptuscamaldulensis, Pseudomonas aeruginosa, exoU, Leaves oil

Abstract

Results of the current study demonstratedthat out of eighty-three isolatesof Pseudomonas aeruginosa,only twenty-five isolateswere resistant to five different antibiotics (of different classes) that were consequentlyconsideredmultidrug resistant isolates.These isolates developed variable susceptibility toward Eucalyptuscamaldulensisleavesoil (ECO). GC-MS analysis of ECOrevealed that the aromatic oil eugenol is the major constituent.However, the most frequent MIC was 0.39 µg/ml, while the lowest frequent MIC was 3.125 µg/ml.Moreover, this oil at ½ MIC (0.195µg/ml) increased the gene expression of exoU. Itis concluded from the outcomes of the studythat ECOmay cause severe damagewhen used to treat infections caused by P. aeruginosa.