Isolation and identification of Citrobacter freundii from chicken meat samples using cultural and molecular techniques

  • Mohammed Hayder Hashim Department of Biology, Al-Farabi University College, Baghdad, Iraq
  • Marwa Hameed AlKhafaji Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq
Keywords: Citrobacter, Chicken, 16S rRNA, Sequencing

Abstract

Because of Citrobacter freundii medical and economical importance and that there are only little local studies about it, this study aimed to isolate and identify this important bacterial species from others that have a similar biochemical and morphological characteristics. Twenty five chicken meat samples were collected randomly from local markets in Baghdad city during 2017; Citrobacter was isolated from the collected samples using selective and differential media and identified using biochemical tests, the identification was confirmed using Vitek 2 compact and polymerase chain reaction for 16S rRNA and the isolated bacteria identified as C. freundii.

Published
2018-07-29
How to Cite
Hashim, M. H., & AlKhafaji, M. H. (2018). Isolation and identification of Citrobacter freundii from chicken meat samples using cultural and molecular techniques. Iraqi Journal of Science, 59(3A), 1216-1224. Retrieved from https://ijs.uobaghdad.edu.iq/index.php/eijs/article/view/462
Section
Biology