EFFECT OF ALCOHOLIC ANASTATICA HIEROCHUNTICA EXTRACTON SOME BIOCHEMICAL AND HISTOLOGICAL PARAMETERS INALLOXAN INDUCED DIABETIC RATS

Abstract

The methanolic extract of Anastatica hierochuntica was investigated for its
antidiabetic effect in male Albino rats. Blood glucose levels, lipids profile were
significantly reduced in all treated group of alloxan diabetic rats in comparison to
their pre-treatment values, in addition to, intake of orally dose of 100 mg/kg b.w of
methanolic extract daily for four consecutive weeks to diabetic rats significantly
decreased the activity levels of all those biomarker liver damage enzymes (S.GOT,
S. GPT and S.ALP), and restored it to normal values. Levels of SOD activity were
significantly decreased in diabetic rats compared with the healthy group, but these
levels increased significantly to a value similar to the healthy rat when treated with
a daily single dose of Anastatica hierochuntica extract ( 100mg/kg b.w) for four
weeks. The same pattern was seen with another antioxidant enzyme GPx, its levels
significantly decreased in diabetic and increased when the animals fed on the extract
daily for four weeks. Histopathological observations in both pancreas and liver rat's
tissues revealed that methanolic Anastatica hierochuntica extract was non-toxic and
regenerated the toxic effects of alloxan. In conclusion the decreased blood glucose
accompanied with decreased lipid profile and changes in the activities of the
antioxidant enzymes SOD and GPx, in addition to biomarker liver damage enzymes
showed the antidiabetic, hypolipidmic and a potent antioxidant agent.