Purification of Crystals Protein by Aqueous Two Phase System fromBacillus thuringiensis Local Isolate

Mohammad  Jaafar; Abdul Karrm Al-Kazaz; Ali  Mohammad

doi:10.24996/

Authors

Mohammad Jaafar Department of Biotechnology, Science Collage, Baghdad University,Baghdad.Iraq.
Abdul Karrm Al-Kazaz Department of Biotechnology, Science Collage, Baghdad University,Baghdad.Iraq.
Ali Mohammad Department of Biotechnology, Science Collage, Baghdad University,Baghdad.Iraq.

DOI:

https://doi.org/10.24996/

Keywords:

Bt,Aqueous,, two phase system,, Protein Purification.

Abstract

The crystals protein of local isolate Bacillus thuringiensis were purified by using
aqueous two phase system method in two steps ; the first 94% of crystals protein and
spores were separated from cell dibbers by using 14% g/ml ammonium sulphate and
4% g/ml of Polyethylene glycol 6000 at pH=7 with separation time of 30 min.
While in the second step, crystals protein were purified from spores by using a
system consisted of 8% g/ml Dextran 60000-90000 and 9% g/ml of Polyethylene
glycol 6000 at pH=7 with separation time of 2.5 h. the a final yield was 70.6% with
purity of 92%