ISOLATION AND IDENTIFICATION SOME THEPATHOGENIC AGENTS FROM ACUTE SUPPURATIVEOTITIS MEDIA (ASOM) IN CHILDREN IN AL-DIWANIYACITY AND THEIR SENSITIVITY TEST AGAINST SOE OFANTIBIOTICS IN VITRO

Abstract

The present study entalid collection of (50) ear swabs from children patients
suffering from Acute suppurative Otitis Media (ASOM) carried at the E.N.T section
of the teaching hospital in AL-Diwaniya a City. Whose ages ranged between (1 day-
5 years) during the Period from October 2006 until Jun 2007. The aim of these study
is to isolate dominant pathogenic causes and responsible of (ASOM) in Children and
effect of some antibiotics on this causes.Staphylococcus aureus bacteria was the
dominant cause of Acute suppurative otitis media with the percentage of 49. 23%.
Followed by Candida albicans with the percentage of 30.76%, Proteus vulgaris
6.15%, Pseudomonas aeruginosa 4.61 B-haemolytic streptococci, Klebsiella
Pneumoniae Haemophilous influeenzae, Staphylococcus epidermidis, Enterobacter
clocase, Moraxella catarrhalis with percentage 1.53% Results showed that 80% of
the infection cases were caused by one pathogenic factor and 20% of them but more
than one factor. The sensitivity test showed that staph. aureus bacteria had the
highest Sensitivity to vancomycin 96.87%, Amikacin 78.12%, Tobromycin 68.74%
and ciprofloxacin 65%, While showed lowest sensitivity to Lincomycin 3.12% while
Proyeus vulgaris showed highest sensitivity to vancomycin 100%, Trobromycin
75%, Amikacin and ciprofloxacin with other antibiotic 50% and showed lowest
sensitivity to Tetracyclin with other antibiotics 25%. Pseudomonas aeruginosa
bacteria showed highest sensitivity to vancomycin and Amikacin 100%. Tetracyclin
and ciprofloxacin with other antibiotics 66.66% while showed lowest sensitivity to
Tobramycin and other antibiotics 33.33%. the results of synergistic antibiotics effect
on Staph aureus showed the effect between Amikacin with (Tobramycin,
ciprofloxacin and tetracycline) had a parentage of 90.47% whilethe synergistic,
effect between Tobromycin and (Amikacin , ciprofloxacin and Tetracycline) hada
percentage of 42.85%.While synergistic effect on Proteus vulgaris showed the effect
between Amikacin with (Tobromycin, ciprofloxacin and Tetracycline) had
percentage of 50%. While synergistic effect between Tobromycin and (Amikacin,
ciprofloxacin and Tetracycline) had a percentage of 66.66%. The results showed that
Candida albicans isolates had the highest sensitivity to Nystatin with inhibition zone
rate (10, 11.8, 18.76) mm, Miconazole (0, 8, 15.3) mm, ketoconazole (10, 9.6,14.94)
mm, Fluconazole (0, 9.6, 12.6) mm, Griseofulvin (0, 0, 6) mm.