EVALUATION OF EFFECT OF EUGENOL PLANT ONTWO OF CANCEROUS CELLULAR LINES (AMN3) ANDNORMAL (REF)

Amina   Al-Thwani; Sanar   Nseaf

doi:10.24996/ijs.2010.51.3.%g

Authors

Amina Al-Thwani Institute of Genetic engineering and Biotechnology for post-graduate studies, University of Baghdad. Baghdad- Iraq.
Sanar Nseaf Institute of Genetic engineering and Biotechnology for post-graduate studies, University of Baghdad. Baghdad- Iraq.

DOI:

https://doi.org/10.24996/ijs.2010.51.3.%25g

Keywords:

EFFECT, EUGENOL

Abstract

The capacity of aqueous and alcoholic extract of Dianthus was studied on two
cell lines, which were: many adino carcinoma (AMN3) and normal cell line of Rat
embryo fibroblast (REF) in vitro and the effect was evaluated of these extracts
It was found that the crude extract of Dinthus contained resins, saponins,
flavonoides, comarins and phenols. Aqueous and alcoholic extract of Dianthus
showed a high inhibition activity on cancer cell lines in all concentrations used and
the effects of Dianthus appeared after 48 hours. From being exposed on the cell in
concentration of 1000 mg/ml.
The highest inhibition result in AMN3 cells were 58.3, 66.7% for both extracts
aqueous and alcoholic and the inhibition effects were similar in both cell line and the
bio results of these cells explained the inhibition effects for the biology of cells
depend on the concentration of crude extract and type of solvent being used.