Brucella melitensis Rev.1 live attenuated Vaccine and its DNA induced IFN- γ and anti-ds DNA antibodies production in rats

Abstract

Brucellosis is one of the five common bacterial zoonoses in the world caused by
organisms belonging to the genus Brucella. Immune recognition of bacterial
infection may contribute to cytokine, as well as antibody production that are
characteristic of innate and adaptive responses. In this study, the presence of
attenuated live Brucella melitenses Rev1 bacteria or its DNA induced the immune
system to produce IFN-γ and anti-ds DNA antibody. In respect to IFN-γ released,
the B. melitensis Rev 1 attenuated live vaccine was able to stimulate the immune
system more than the DNA (P≤0.05). Such finding could be attributed to the whole
attenuated bacteria that have immunogenic factors other than the DNA like cell wall
component and outer membrane. On the other hand, the B. meliensis Rev 1 DNA
activated the B cell to secret anti-ds DNA antibodies significantly higher (P≤ 0.05)
than live attenuated vaccine, and the level of antibodies was increased to parallel the
concentration increases of injected DNA.