Histopathological Effects of Cadmium Chloride on Barbus sharpeyi

Abstract

The present study including determined pathological changes in Barbus sharpeyi, as
well as description behavior and growth of bunni fish by used 180 fingerlings, Fish
were distributed randomly upon four treatments in addition to control group. First
treatment (T1) contained cadmium 0.093mg/L with changing water and added cadmium
continuously, the second treatment (T2) contained cadmium 0.093mg/L with changing
water without adding cadmium, third treatment (T3) contained cadmium 0.046mg/L
with changing water and adding cadmium continuously, fourth treatment (T4) contained
cadmium 0.046mg/L with changing water without adding cadmium. In order to estimate
LC50 used 120 fingerlings of fish B. sharpeyi, were exposed to 0.5, 0.6, 0.7, 0.8, 0.9
and 1.0mg/L. The LC50 of cadmium was 0.932mg/L for 48h of exposure. Fish behavior
was recorded showed abnormalities after exposure to the various cadmium
concentrations such as swimming disorders, the fish tended together at the surface, fast
movement, fish aggregate in aquarium border, fish weakness, opened and closed in
operculum festally, as well as a significant decrease at (P≤0.05) in body weight of
T1,T2 and T3. Histopathological changes in gills characterized by lamellar fusion,
lifting of secondary lamella, The main findings in liver tissue were hydropic swelling
and fatty degeneration of some hepatocytes, focal or diffuse necrosis, Kidney result
varying degrees of tubular necrosis with severe congestion together with
melanomacrophage infiltration. Intestine results showed slauphing and necrosis of
mucosal epithelial of intestinal villi and diffuse MNCs infiltration, hyperplasia and
hypertrophy of goblet cell. Spleen observed severe destruction in spleenic parenchyma,
severe reduction in hemopoitic tissue.