Study the Prophylactic Role of Anti-Type IV Pili (fimbriae) Antibody Against Pulmonary Infection Caused by P.aeruginosa in vivo (mice)

Abstract

This study was aimed to investigate the effect of anti- type 4 pili antibody in
prevention pulmonary infections caused by P. aeruginosa in vivo. This was
achieved by Evaluation of biofilm formation by the microtiter plate method to
select P.aeruginosa isolate with highest biofilm formation capacity, Extraction
and the partial purification of type IV pili from the selected isolate, then
Preparation of type IV pili antibodies by rabbit immunization. The lung
histological sections of non immunized mice were severly damaged ,while the
damage were markedly decrease in the lung of immunized mice with anti-type 4
pili antibody.