Phylogenetic analysis of cpn60 gene from locally isolated Acinetobacter baumannii

Abstract

This study was aimed to analysis phylogenetic tree of the gene cpn60 in Acinetobacter baumannii that was identified in Baghdad. Study included collection two hundred specimens (fifty from UTI, fifty from wound infection , fifty from respiratory tract infection and fifty from otitis infections) . In primary laboratory diagnosis and confirmed by using VITEK- 2 Compact system, twenty isolates of this bacterium were indentified (10%) from total specimens. Extraction of geneteic material to detect target gene by amplification this target gene. DNA
sequencing of all isolates was done. Then alignment of sequencing in NCBI and draw phylogenetic tree by use Geneious 9 software among sequence of locally isolates . The results in phylogenetic tree of cpn60 gene in locally isolates showed 4 groups of isolates different with difference source of isolation. Then phylogenetic
tree for locally isolates and high identity global isolates in gene bank was drew and its results showed 12 locally isolates not identity with standard isolates. So, chosen isolate (AE_29) isolate from these 12 isolates and documented in national GenBank as anew isolate under accession number (LOCUS KY818056 ) of nucleotides
sequence and protein ID "ARV90994.1" .