Evaluation of Rosmarinus officinalis Leaves Essential Oils Activity Against Vancomycin Intermediate Staphylococcus aureus (VISA) Isolated from Baghdad Hospital Patients

Authors

  • Osamah Z. Al-Hayali Department of Biology, Al-Farabi University College , Baghdad, Iraq
  • Mohamed. F. AL Marjani Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq
  • Abbas Maleki Clinical Microbiology Research Centre, Ilam University of Medical Sciences, Ilam, Iran

DOI:

https://doi.org/10.24996/ijs.2023.64.5.5

Keywords:

Essential oil, Antimicrobial activity, GC-MS, Real-time PCR

Abstract

      Rosemary is a well-known aromatic and medicinal plant used to treat various ailments. This study evaluated Rosmarinus officinalis essential oil for its phytochemical and antibacterial properties. The essential oil was analysed by using a gas chromatography-mass Spectrometry (GC-MS) that revealed the common chemicals containing verbenone 36.20% and 1,8-cineol (Eucalyptol) 12.14%. Extracted essential oils were tested for antibacterial activity against vancomycin intermediate Staphylococcus aureus (VISA), a strain of bacteria obtained locally from bacteremia patients. Three isolates were found to be VISA positive using the E-test (strips) and the population analysis profile method (PAP). VISA showed lower resistance to the essential oils (MIC: Minimum inhibitory concentration, MIC = 1.5 mg/ml). Overall, the genetic influence of VISA was evaluated by looking at the hld gene expression after being isolated from blood samples and RNA extraction for qRT-PCR to determine hld gene expression. R. officinalis gene expression was analysed and calculated by using real-time PCR of RNA which revealed that it had decreased.

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Published

2023-05-30

Issue

Section

Biology

How to Cite

Evaluation of Rosmarinus officinalis Leaves Essential Oils Activity Against Vancomycin Intermediate Staphylococcus aureus (VISA) Isolated from Baghdad Hospital Patients. (2023). Iraqi Journal of Science, 64(5), 2153-2167. https://doi.org/10.24996/ijs.2023.64.5.5

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