Spectrophotometric Determination of Metoclopramide Hydrochloride in Pharmaceutical Formulations Using Diazotization Coupling Reaction

Rabee Mohammed Ali Yassin; Nabeel Sabeh Othman

doi:10.24996/ijs.2023.64.9.1

Authors

Rabee Mohammed Ali Yassin Department of Chemistry, College of Science, University of Mosul, Mosul, Iraq
Nabeel Sabeh Othman Department of Chemistry, College of Science, University of Mosul, Mosul, Iraq https://orcid.org/0000-0002-5930-3925

DOI:

https://doi.org/10.24996/ijs.2023.64.9.1

Keywords:

Spectrophotometric, metoclopramide hydrochloride, 1,7-dihydroxynaphthalene diazotization, coupling reaction

Abstract

For the determination of metoclopramide hydrochloride (MCPD) in pharmaceutical formulations, a rapid and straightforward spectrophotometric method has been proposed. The method involves diazotizing the main amino group of MCPD with sodium nitrite followed by coupling reaction with reagent 1,7-Dihydroxynaphthalene (1,7-DHN) to form a stable and colored compound in alkaline medium of sodium hydroxide which showed a maximum absorbance‎ intensity at the wavelength 578 nm. The linearity of developed method has ranged from 1.0 - 15 ‎ µg.ml^-1‎‎ while the molar absorptivity 2.9867x10⁴ l.mol^-1.cm^-1, RSD% was less than 1.11%. While the LOD and LOQ were 0.059 ‎µg.ml^-1‎‎ and 0.198 ‎ µg.ml^-1‎‎ respectively. The method is appropriate for the determination of MCPD in the presence of other substances that are typically included in dosage forms.