Molecular Investigation of Some Beta-lactamase Genes by PCR and DNA Sequencing Techniques in clinical Escherichia coli

Rana AH AL-Lami; Huda S. A. Al-Hayanni; Zina Hashem Shehab

doi:10.24996/ijs.2022.63.10.7

Authors

Rana AH AL-Lami Biology Department, College of Science for women, University of Baghdad, Iraq
Huda S. A. Al-Hayanni Biology Department, College of Science for women, University of Baghdad, Iraq
Zina Hashem Shehab Biology Department, College of Science for women, University of Baghdad, Iraq https://orcid.org/0000-0002-9730-8380

DOI:

https://doi.org/10.24996/ijs.2022.63.10.7

Keywords:

Escherichia coli, blaTEM and blaCTX-M genes, antibiotic resistance, PCR, nucleotide sequencing

Abstract

In this study, out of 50 isolates of some nosocomial infections from some Baghdad hospitals, only 13 (26%) were identified as Escherichia coli. Depending on selective media, morphological and biochemical tests the species was then confirmed by molecular methods. Later on antimicrobial resistance test was performed by the Kirby-Bauer method. The molecular characterization of blaTEM and blaCTX-M genes in different clinical isolates of E. coli was done through polymerase chain reaction (PCR) by utilizing special primers. These genes were positive to only 4 (30.7%) isolates. The sequence of nucleotides of positive genes was carried out for four isolates. The results showed that there was no variance in the nucleotide sequence between Iraqi isolates compared with the global isolates, and that they were 100% identical to many genera of Enterobacteriaceae. Finally, due to the indiscriminate use of antibiotics, these genes in human strains were likely the source of widespread drug resistance.