Antibiofilm activity of klebocin crude extract against some species of Enterobacteriaceae

Abstract

Bacteriocins are antibacterial proteins created by bacteria and its effective against   other strains of bacteria which are closely linked to the producing strains and a number of species from the same family. The aim of current study was to evaluate the activity of   bacteriocin  that extracted from  clinical  isolates of Klebsiella pneumonia against different  species of enterobacteriaceae in both planktonic and biofilm state. The antibacterial activity of bacteriocins (klebocins) from Klebsiella pneumoniae isolates  towards diverse pathogenic species  from enterobacteriaceae (by  well assay method) was demonstrated , however   the antibiofilm activity against some species of enterobacteriaceae  was studied also by   Tissue culture plate method (TCP) in first and last stages of biofilm formation .The results  revealed that klebocin with different concentrations  was efficient against  different pathogenic species by producing different inhibition zones. Results also showed that klebocins had a wide antibiofilm activity on  some pathogenic species of Gram-negative bacteria .In addition to these  finding it was observed that the antibiofilm activity of klebocin  on premature  biofilm of Klebsiella was higher than  its effect towards other  pathogenic species, while it was affected  on mature biofilm of other bacterial species as well as Klebsiella.