Analysing Aflatoxin Production Conditions in Feed Samples Using a Preparative Thin Layer Chromatography (TLC) Method

Authors

  • Sarra A. Abrahem Department of Chemistry, College of Science, Al-Nahrain University, Baghdad, Iraq

DOI:

https://doi.org/10.24996/ijs.2022.63.1.2

Keywords:

B1 aflatoxin, F.sporotrichoides, A.parasiticus, TLC method

Abstract

    In this research, aflatoxins were produced, extracted, isolated, and purified in order to optimize the storage conditions of feed. Using a preparative thin layer chromatography (TLC) method, with commercially available plates of 1.5 to 2.0 mm, B1 aflatoxin was isolated from the feed samples of whole wheat, maize, and crushed rice, and the procedure was repeated four times. A purity value of 99 % for B1 aflatoxin was achieved and tested using spectrophotometric and chromatographic methods. As solvents, acetone:water (85:15l) was used for aflatoxin extraction from the feed sample, whereas methanol: water (50: 50) was used for trichothecenes extraction. The primary findings of this research indicate that B1 aflatoxin reached a maximum yield when coarsely crushed rice was inoculated with  Aspergillus parasiticus at a temperature of 30C°, moisture level of 35%,  and incubation duration of 7 days. The observations obtained from aflatoxins production process provide an insight regarding storage conditions that need to be avoided in order to reduce the incidents of feed contamination.

Downloads

Download data is not yet available.

Downloads

Published

2022-01-30

Issue

Section

Chemistry

How to Cite

Analysing Aflatoxin Production Conditions in Feed Samples Using a Preparative Thin Layer Chromatography (TLC) Method. (2022). Iraqi Journal of Science, 63(1), 9-20. https://doi.org/10.24996/ijs.2022.63.1.2

Similar Articles

1-10 of 989

You may also start an advanced similarity search for this article.