Assay of Orphenadrine Citrate in Pharmaceuticals via Extraction-Spectrophotometric Method

  • Lazeeza Sattar Omer Department of Pharmaceutical Chemistry, College of Pharmacy, Hawler Medical University, Erbil, Iraq
  • Rasul Jameel Ali Department of Clinical Biochemistry, College of Health Sciences, Hawler Medical University, Erbil, Iraq
Keywords: Orphinadrine citrate, Paracetamol, Ion-pair complex, Eriochrome black T

Abstract

An extraction-spectrophotometric technique has been applied and approved for the estimation of orphenadrine citrate in the presence of paracetamol in a binary synthetic mixture and in combined drugs. The procedure is built on the formation of a soluble red colour orphenadrine citrate – eriochrom black T (EBT) ion pair complex at pH 1.40, while the paractamol not paired. The produced red colour ion-pair complex was extracted with chloroform and showed maximum absorption at 509 nm. For quantitative evaluation Beer’s law applied to plot the absorbance against concentration, the relation was a linear in the concentration range of 0.10-6.00 µg/ mL with the molar absorptivity 4.4025 x104 L / mol. cm. The limit of detection and limit quantification were 0.024 µg/ mL, and 0.100 µg/ mL respectively. The intra-assay precision evaluated in terms of % relative standard deviation (RSD) (< 2%). And accuracy was validated with % recovery (98.8-102.5%). The results showed that orphenadrine citrate could be determined successfully in the combined tablet without interference by paracetamol and other common co-formulated substance.

Published
2018-07-29
How to Cite
Omer, L. S., & Ali, R. J. (2018). Assay of Orphenadrine Citrate in Pharmaceuticals via Extraction-Spectrophotometric Method. Iraqi Journal of Science, 59(3A), 1152-1161. Retrieved from https://ijs.uobaghdad.edu.iq/index.php/eijs/article/view/372
Section
Chemistry