Antibiotic Resistance Patterns of Bacteria Isolated from Dechlorinated Water Samples

Abstract

Chlorination has been the method of choice for disinfecting water used for drinking purposes. However, some stressed bacteria during chlorination are able to recover and alter the potability of water. This study assessed the recovery of stressed bacteria in dechlorinated water. Ten chlorinated water samples were collected from different points within Ilorin metropolis, Kwara, Nigeria. The samples (100ml) were dechlorinated with 0.1ml of 11.4mM sodium thiosulphate solution. The physicochemical characteristics of the chlorinated water samples were determined while bacteriological analyses were carried out on both chlorinated and dechlorinated water samples. The antibiotic susceptibility pattern of the isolates was determined using disc diffusion method. The physicochemical characteristics of the water samples ranged as follow: pH 7.3-8.4, chloride content 4.37-6.85 mg/l, suspended solids 0.004-0.017 g/100ml, and total hardness 30-72mg/l. The chlorinated water samples had bacterial, total, and faecal coliform counts ranging from 1.0 × 10¹ – 1.9 × 10⁴cfu/ml, 0 – 480 MPN/100ml, and zero, respectively. The dechlorinated water sample had corresponding counts of 5.4 × 10² – 7.36 × 10⁴cfu/ml, 6 - 1100 MPN/100ml, and 0 – 380 MPN/100ml. A total of eleven bacterial species belonging to the genera Bacillus, Burkholderia, Citrobacter, Enterobacter, Enterococcus, Escherichia, Staphylococcus, Serratia, and Streptococcus were isolated. Not lower than 60% of the bacterial isolates were susceptible to ofloxacin and ciprofloxacin. All the isolates exhibited multiple antibiotic resistances.  The antibiotic resistance pattern of an isolate of Citrobacter freundii to cefuroxime, cefixime, and gentamycin changed remarkably and was plasmid-mediated, while that of E. coli and Enterobacter agglomerans remained unchanged to all the antibiotics and was non-plasmid mediated. Chlorination of water at the point of use is recommended. It is concluded that chlorination is essential in order to prevent reactivation of stressed bacteria during distribution and prevent infection by bacteria with high multiple antibiotic resistance index.