Genetic Polymorphisms and Haplotype Analysis of Endothelin-1 Gene in Diabetes Mellitus Type II Patients

Abstract

Introduction: Endothelin-1gene is an important factor that can contribute to cardiovascular and kidney disorders. Numerous single nucleotide polymorphisms (SNPs) in the Endothelin-1gene was related to many diseases and may be associated with complications of diabetes mellitus type II.This study aimed to investigate the relationship between Endothelin-1genetic polymorphism and DM type II in the Iraqi population.

 Methods: Blood samples were collected from patients with type II diabetes and control. PCR was applied to detect the Endothelin-1gene and conducted genetic sequences for Endothelin-1gene to detect SNPs (rs1800543, rs2070699, rs1476046, rs1762831452, new671, rs587777231, rs587777234). Results: SNP rs1800543 showed that the odds ratio for genotype CC (1.52) and C allele (1.12) both consider as an etiological fraction positively associated with diabetic type II. SNP rs2070699.The results showed that the odds ratio for genotype GG (1.8) and G allele (1.35) were associated with a positive correlation of diabetic type II as an etiological factor. The results of rs1476046 showed that the odds ratio for the genotype GA and A allele were 1.42 and 1.22, indicating an etiological fraction as a positive association with diabetes. According to the result linkage disequilibrium, the haplotype CCTAT, TAGGC, TATGC, and TCTAT represent a risk factor that makes an individual more susceptible to diabetes mellitus type II. In conclusion, SNPs (rs1800543, rs2070699, rs1476046, rs1762831452, and new 671) affect more susceptibility to diabetes. The linkage disequilibrium results in the current study showed a strong association between rs2070699 and rs1476046 SNPs. The haplotype TAGGC represents the widespread haplotype with risky effects, making individuals more susceptible.