CHARACTERIZATION OF ANTIBACTERIAL SUBSTANCEPRODUCED BY Pseudomonas Fluorescens

Authors

  • Sinai Waleed Tropical - Biological Research Unit, College of science. University of Baghdad. Baghdad-Iraq.

DOI:

https://doi.org/10.24996/ijs.2009.50.2.%25g

Keywords:

ANTIBACTERIAL, Pseudomonas

Abstract

Pseudomonas fluorescens isolate was isolated from soil. The antibacterial activity of crude extract of this isolate was determined against pathogenic bacteria (Klebsiella pneumonia, Staphylococcus aureus) which are isolated from patients with skin infections. The extraction of this substance was done and study it characterization after purified by silica gel chromatography, It was stable within range of pH (5-9) at 37 ºC for 24 hr. and inactivated by serum at concentration > 50%. The substance was soluble in methylene chloride, chloroform, acetone, sodium hydroxide and hydrochloric acid and it was insoluble in water, methanol, ethyl acetate and hexane.

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Published

2024-10-17

Issue

Section

Biology

How to Cite

CHARACTERIZATION OF ANTIBACTERIAL SUBSTANCEPRODUCED BY Pseudomonas Fluorescens. (2024). Iraqi Journal of Science, 50(2), 267-270. https://doi.org/10.24996/ijs.2009.50.2.%g

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