CYTOTOXICITY EFFECT OF AQUEOUS EXTRACT OF BRASSICA RAPA ROOTS ON CANCER CELL LINES IN VITRO

Nadia   Barakat; Hind    Obaid; Amal   Ali; Aiman   Hassan; Zainab   Abaas

doi:10.24996/ijs.2010.51.4.%g

Authors

Nadia Barakat University of Al-Mustansiyria ,Iraqi Center of Cancer and Genetic Medicine,Baghdad , Iraq.
Hind Obaid Department of Biology, College of Science,University of Baghdad, Baghdad - Iraq.
Amal Ali University of Al-Mustansiyria ,Iraqi Center of Cancer and Genetic Medicine,Baghdad , Iraq.
Aiman Hassan University of Al-Mustansiyria ,Iraqi Center of Cancer and Genetic Medicine,Baghdad , Iraq.
Zainab Abaas Department of Biology, College of Science,University of Baghdad, Baghdad - Iraq.

DOI:

https://doi.org/10.24996/ijs.2010.51.4.%25g

Keywords:

EFFECT, AQUEOUS

Abstract

Effect of aqueous extract of Brassica rapa roots on three types of cancer cell lines; Hep-2, AMN-3 and Hela was studied in vitro.
The results showed that, the cytotoxic effect of extract dependent on type of cells, amount of dose and exposure time.
The concentration 1250 μg/ml gave higher growth inhibition , were ( 63 and 42 ) % to ANM-3 and Hep-2 respectively comparied with control 100% after 24 hours from exposure time , but inhibition gowth of Hela cells was 64% by 10000 μg/ml crud roots extract for 24 hours.
Exposure time for 48 hours reduced the percentage of inhibited growth٫ only by
10000 μg/ml for extract; viability was 25 % for ANM-3 cells.
Crud aqueous extract of Brassica rapa roots was have hormetic effect ( Hormesis ), because also induced the proliferation of cancer cells by used low concentration of extract after 72 hours exposure time.