IDENTIFICATION OF DIFFERENCES IN VIRULENCEFACTORS PRODUCTION FROM UV IRRADIATED ISOLATEOF CLINICAL Vibrio cholerae S

Abstract

Mutants from the clinical isolate of Vibrio cholerae S were isolated after UV
irradiation on the basis of antibiotic resistance for Rifampcin, Streptomycin and
Klindamycin. Results revealed that the highest antibiotic resistant incidence is for
Rifampicin after UV exposure for 15 minute.
One thousand mutant isolates were examined and showed smaller sized colonies on
TSA appeared as smooth and translucence with yellow color on TCBS agar and pale
on MacConkey agar. The mutant isolates gave positive results with oxidase and
string test. Autoagglutination range from high level order designated +++ to mild ++
and low level designated as +. Only 15% of Rifampicin resistant mutant isolates
gave no agglutination phenomenon. UV treated isolates produced delayed type of
proteases with no changes in haemolycin and lipases production while, slightly
autoagglutination has been detected from these mutant. Twenty out of screened
isolates produced 10-30U/ml of CT at non- permissive conditions.
The toxin extract from mutant isolates symptoms such as muscle spasm, muscle
cramp, exhaustion, trummer and tachycardia in mice after intraperitoneal injection.
Erythemal effect and induration in the injected site of Guinea pig skin after
intradermal injection remained even after more than one week, with pronounced
bleeding detected in the injected area.