PLASMID CURING OF LOCAL ISOLATES OF KLEBSIELLAPNEUMONIAE ISOLATED FROM URINARY TRACTINFECTIONS AND ITS ROLE IN MULTIDRUG RESISTANCE

Abstract

Curing of plasmid for 7 local isolates of K. pneumoniae have multi-drug resistance
and isolated from urinary tract infections (UTI) by using acridin orange and sodium
dodecyl sulphate (SDS) were investigated.
These results showed that the local isolates of K. pneumoniae were very resist
(100%) to Ampicillin, Pepracillin, Amoxicillin, Penicillin G, Tetracyclin,
Erythromycin and Gentamycin, high resist (71–80%) to Cephalexin, Cephalothin,
Ceftazidine, Ciprofloxacin, Streptomycin and Tobramycin, moderate resist
(43–57%) to Cefaclor, Cephradin and Naldixic acid and weak resist (29%) to
Cefotaxime while they were susceptible to Chloramphenicol and Amikacin.
t was found that SDS is more efficient in curing than Acridin orange, hence the
losing of the resistance of antibiotics by using SDS was in the range (70–100%)
while by using Acridin orange was in the range (28– 100%). After curing of plasmid
the Antibiotics resistance losed for Pipracillin, Amoxicillin, Penicillin G,
Cephalexin, Cephalothin, Ceftazidin, Tetracyclin, Gentamycin and Tobramycin, this
result confirm that the resistance of these antibiotics is born on plasmid, while the
resistance of Ampicillin, Ciprofloxacin, Erythromycin and Streptomycin not losed,
this result confirm that there resistance genes are born on the chromosome.