STUDY THE EFFECT OF PURIFIED CATECHINS OF GREEN TEA ONTHE MOST IMPORTANT CAUSES OF BACTERIAL INFECTIONS OFTHE URINARY TRACT AND SYNERGISTIC EFFECT

ESSAM   Al-jumaily; Yasir   Al-Abdli; Nazar   Nasser

doi:10.24996/ijs.2011.52.3.%g

Authors

ESSAM Al-jumaily Department of Biotechnology, Institute of Genetic Engineering and Biotechnologyfor post graduate Studies, University of Baghdad. Baghdad- Iraq
Yasir Al-Abdli Department of Biology, College of Science, Unversity of Al-Mustansirya. Baghdad - Iraq
Nazar Nasser Department of Biology, College of Science, Unversity of Al-Mustansirya. Baghdad - Iraq

DOI:

https://doi.org/10.24996/ijs.2011.52.3.%25g

Keywords:

CATECHINS, GREEN

Abstract

Two hundred urine samples were collected from patients infected with acute and
chronic urinary tract infection, 85 bacterial isolate were isolated from these samples
and were diagnosed using biochemical tests and kit API test, most of them identified
E.coli (23.5%) K.pneumoniae rate (17.6%). Isolates were selected E.coli and
K.pneumoniae according to their isolation rate from urinary tract infection cases and
their antibiotic resistance. The inhibition effect of the purified catechin from the
green tea was studied when the isolates were grown in the following concentrations
(25, 50,75, 100) mg/ml. The concentration of 100 mg/ml showed a high action
against the two species. The combination effect of the purified catechin was
estimated with both Amikacin and Gentamycin on the resistant isolates to these
antibiotics (E.coli and K.pneumoniae) and has been compared to the minimum inhibitor concentration of the isolates before and after the confusion of antioxidants,
as the effect of mixing Amikacin and Gentamycin with Catechin, it gave a synergic
effect with Amikacin on two isolates, either Gentamycin gave synergic effect on
K.pneumoniae.