EFFECT OF SOME CHEMICAL AND PHYSICAL FACTORS ON THECOCCI SHAPE FORMATION OF HELICOBACTER PYLORIISOLATED FROM PATIENTS WITH DUODENUM ULCER

Abstract

Helicobacter pylori was islotaed localy from patients with Duodenal Ulcer. Two
biopsies were taken from each patient, the first used for isolation of H. pylori using
ski row medium, the second used for rapid urease test. Optimum growth temperature
was 37 c. Isolated H. pylori was identified according to bacterial shape, graw stain,
biochemical test, sensitivity to cephalothin & resistance to nalidixic acid. The result
showed that component of culture media, light, & oxygen are important factors affecting Hansformed from spiral to coccoid shapes. It has been noticed that using
Brain – heart infusion a gar and blood a gar base with growth supplement give &5%
blood, percentage transformation to coccoid shape was less than using the same
culture media without blood and growth supplements. Results indicated that
avoiding exposure of culture media to light and oxygen (more than 5%) maintain
spiral shape of H. pylori for 20 – 30 days when stored at 4 c taking in consideration
a pH of (6.9 – 8).