Purification of Crystals Protein by Aqueous Two Phase System fromBacillus thuringiensis Local Isolate

Authors

  • Mohammad Jaafar Department of Biotechnology, Science Collage, Baghdad University,Baghdad.Iraq.
  • Abdul Karrm Al-Kazaz Department of Biotechnology, Science Collage, Baghdad University,Baghdad.Iraq.
  • Ali Mohammad Department of Biotechnology, Science Collage, Baghdad University,Baghdad.Iraq.

DOI:

https://doi.org/10.24996/

Keywords:

Bt,Aqueous,, two phase system,, Protein Purification.

Abstract

The crystals protein of local isolate Bacillus thuringiensis were purified by using
aqueous two phase system method in two steps ; the first 94% of crystals protein and
spores were separated from cell dibbers by using 14% g/ml ammonium sulphate and
4% g/ml of Polyethylene glycol 6000 at pH=7 with separation time of 30 min.
While in the second step, crystals protein were purified from spores by using a
system consisted of 8% g/ml Dextran 60000-90000 and 9% g/ml of Polyethylene
glycol 6000 at pH=7 with separation time of 2.5 h. the a final yield was 70.6% with
purity of 92%

Downloads

Download data is not yet available.

Downloads

Published

2024-03-26

Issue

Section

Biotechnology

How to Cite

Purification of Crystals Protein by Aqueous Two Phase System fromBacillus thuringiensis Local Isolate. (2024). Iraqi Journal of Science, 53(4), 788-806. https://doi.org/10.24996/

Similar Articles

1-10 of 1046

You may also start an advanced similarity search for this article.