EXTRACTION AND PARTIAL PURIFICATION OF LIPOPOLYSACCHARIDE FROM KLEBSIELLA OXYTOCA AND STUDY OF ITS ROLE IN THE PATHOGENICITY

Abstract

K.oxytoca bacteria was isolated from patients with urinary tract infection
[UTI]from both sexes and at different ages and were identified by biochemical tests
.the isolates were sensive to Amikicin Nitrofurantion and Ticarcillin \Clavulinic acid
and resistant to β-lactam antibiotics The isolate that gave highest percentage of
resistant to antibiotics was used for LPS extraction by phenol-hot water method.
LPS partial purification by gel filtration on Sepharose Cl-6B-200 showed that the
percentage of carbohydrate in the partially purified LPS was 26.6% and percentage
of binding protein was 0.06% and no nucleic acid was found. The LD50 of the
bacteria and LPS was determined by injection of the male mice, it was 3.16X 106 cfu
for bacteria and 266 μg/ml for LPS.