The Role of Antibiotic-Killed Staphylococcus aureus and Its DNA to Cause Arthritis in Rats

Abstract

The purpose of this study is to investigate the capability of bacterial DNA
compared to bacterial lysate in stimulating arthritis using rat model. One hundred
mid-stream urine specimens were collected during November 2012 to January 2013,
from patients suffering from urinary tract infections attending hospitals in Baghdad,
Iraq. Susceptibility of Staphylococcus aureus isolates to antibiotics was examined.
Twenty five isolates were identified as S. aureus and they developed multi drug
resistance. S. aureus S1 lyaste and its DNA were intra-articulary injected in rats. The
levels of IL-6, anti-ds DNA Ab and leukocytes count were measured. In general, IL-
6, anti-ds DNA Ab and leukocytes count were significantly higher in sera of rats
injected with cell lysate. Moreover, time period significantly (P< 0.05) affected the
immunological aspects and joints damage. In a conclusion, bacterial lysate caused
more damage to rat joints than DNA. Furthermore, bacterial lysate evoked
immunological parameters more than DNA did.