Using ISSR markers to build a phylogenetic of Barley Genotypes

Abstract

This study is attempts to build a phylogenetic between nine Iraqi barley
genotypes based on ISSR-PCR analysis by determine the level of genetic similarity
among them. Nine issr primers used in this study produced 41 bands across nine
studied varieties. Of these bands, 28 bands were polymorphic and the remaining
monomorphic bands were 13. The average polymorphic rate was 70.5% ranged
between 25%-100% , and average of polymorphic bands /primer was 4.5.The size
of the amplified bands ranged 140-1600 bp. It was generated a 5 unique bands in
this study, these bands can be used as a DNA profiling of all studied genotypes. The
results were showed Genetic distances ranged between (0.0854-0.9897) among
barley varieties. Based on phylogenic tree, a dendrogram were constructed among
studied barley varieties, cluster analysis grouped the nine varieties into two main
clusters depending on their ancestors and their morphological traits. The use of
cultivars from various clusters and sub clusters offer the possibility of obtaining an
appropriate genetic variability in hybrid population.