Evaluation of the Efficacy of Different Serological Methods (rK39 dipstick, ELISA and IFA) Techniques in the Diagnosis of Visceral leishmaniasis.

Authors

  • Israa S. Muosa Department of Biology, College of Science, University of Baghdad. Baghdad, Iraq
  • Nada N. Younis Department of parasitology, Central Heath Laboratory, Baghdad, Iraq
  • Wasan E. Jawad Department of parasitology, Central Heath Laboratory, Baghdad, Iraq

Keywords:

Visceral leishmaniasis, rK39 Dipstick, ELISA, IFA

Abstract

The objective of this study was to compare the sensitivity and specificity of rK39,
ELISA and IFA tests for the diagnosis of visceral leishmaniasis using blood
samples. Samples were collected from 146 suspected patients and 30 healthy
individual as control. The results showed 52 (35.62%), 58 (39.73%) and 71
(48.64%) positive samples by rK39, ELISA and IFA , respectively. IFA test showed
a higher sensitivity (48.63%) than ELISA and rk39 dip stick (39.72%) and (35.61%)
respectively. The highest sensitivity of agreement (K: 0.912) was found between
rK39 dipstick and ELISA. Therefore, we suggest the application of rK39 dipstick
and ELISA methods in endemic areas of visceral leishmaniasis for early and
accurate diagnosis of this disease

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Published

2023-03-30

Issue

Vol. 56 No. 3B (2015)

Section

Biology

How to Cite

Evaluation of the Efficacy of Different Serological Methods (rK39 dipstick, ELISA and IFA) Techniques in the Diagnosis of Visceral leishmaniasis. (2023). Iraqi Journal of Science, 56(3B), 2218-2222. https://ijs.uobaghdad.edu.iq/index.php/eijs/article/view/9758

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