RP-HPLC Method for Simultaneously Quantifying the Antiviral Drug Contents of Acyclovir, Amantadine, and Oseltamivir in Pharmaceutical Formulations

Authors

  • Sohair Salah Ahmed Department of Chemistry, College of Science, University of Baghdad, Al-Jadriya campus, 10071 Baghdad, Iraq
  • Ashraf Saad Rasheed Department of Chemistry, College of Science, University of Baghdad, Al-Jadriya campus, 10071 Baghdad, Iraq https://orcid.org/0000-0001-9958-7582

DOI:

https://doi.org/10.24996/ijs.2024.65.3.2

Keywords:

RP-HPLC, antiviral drugs, oseltamivir, acyclovir, amantadine

Abstract

     Antibiotics treat bacterial infections, whereas antiviral drugs treat viral illnesses. Antivirals are used to treat a variety of infectious diseases, including COVID-19 and influenza. The technique of reversed-phase high-performance liquid chromatography (RP-HPLC) provides more sensitivity and precision than other approaches, particularly spectrophotometric. This research seeks to develop a simple method for simultaneously quantifying acyclovir, amantadine, and oseltamivir in creams and capsules. The RP-HPLC method optimization and development for verifying the separation and quantification of three antiviral drugs included the investigation of the optimal buffer concentration, pH value, and acetonitrile content. On a C8 HyperClone BDS column, the RP-HPLC system with UV detection achieved separation (250 x 4.60 mm, 130A, and 5). The mixture of acetonitrile and acetate buffer as mobile phase gradient elution at a detection wavelength of 254 nm and 1 mL/min flow rate. The proposed method provided a linear range of 0.08-10.5, 0.06-4.5 and 0.02-14 μg/mL, as well as excellent validated values for LOD (0.0205, 0.0107, and 0.0083 μg/mL) and LOQ (0.0621, 0.0324 and 0.0251 μg/mL) with a coefficient of determination (r2) of the regression line of 0.9998, 0.9988, and 0.9994 for acyclovir, amantadine, and oseltamivir, respectively.

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Published

2024-03-29

Issue

Section

Chemistry

How to Cite

RP-HPLC Method for Simultaneously Quantifying the Antiviral Drug Contents of Acyclovir, Amantadine, and Oseltamivir in Pharmaceutical Formulations. (2024). Iraqi Journal of Science, 65(3), 1188-1199. https://doi.org/10.24996/ijs.2024.65.3.2

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