Identification of Antibiotic-Resistant Genes in Salmonella Typhi Isolated From Typhoid Patient in Samawa City

Abstract

A total of 100 blood samples taken from patients with suspected typhoid fever aged between (1-60) years, were involved in this study. Blood samples were cultured directly on brain heart infusion broth. After that sub cultured of isolates on MacConkey agar and XLD agar and S.S agar to find the Salmonella typhi then identified by the biochemical and antibiotic sensitivity test. Resistant genes were identified by using aacc2 gene and cat gene. Results showed that there was 7 Salmonella typhi isolates from blood culture, as well as, aacc2 gene success in amplification of 450bp fragment for amino glycoside resistant, while not improve amplification for chloramphenicol resistant gene (cat gene) from Salmonella typhi isolates . also our isolates showed sensitivity 100% for ceftriaxone, cefepime, cefazolin, and chloramphenicol, while all isolates were resistant 100% for tetracycline and erythromycin.