Effect of Some Materials on Productivity and Activity of -Hemolysin which Produced by Methicillin Ressistant Staphylococcus Aureus

Authors

  • Khawlah Jebur Khalaf Department of Biology, College of Science, Al- Mustansiriya University, Baghdad, Iraq
  • Zainab Farhan Ali Department of Biology, College of Science, Al- Mustansiriya University, Baghdad, Iraq

Keywords:

-hemolysin, Methicillin- ressistant Staphylococcus aureus, CD-Cholesterol, Cholesterol, Cyclodextran (CD), Methicillin and PBS

Abstract

In this study 77 human isolates of Staphylococcus aureus were obtain from different clinical sources. The results showed that the number of isolates producing α-hemolysin was 32 isolates (41.55%) , while non- hemolysin producing was 45 isolates (58.45%) .The minimum inhibitory concentration (MIC) of methicillin which were 32 micrograms \ ml. The effect of CD-Cholesterol ,Cholesterol ,Cyclodextran(CD) ,Methicillin and Phosphate Buffer Saline(PBS) on α- hemolysin activity was study and the hemolytic Titer was:8 ، 32.768 ,65.536 ,
140.737.488.355.32 , 4.961408E + 25 respectively, while the effect of the same effect with Titer 67.108.864 and low Titer with PBS 3.96140812E + 25. The α – hemolysin toxin was partialy purified by ammonium sulfate precipitation. partial purified toxin was injected inside the cornea of rabbit's eye and the erosion of cornea formed with diameter: 8 mm after 8 hours of injection while the erosion of cornea which injected with CD-Cholesterol that mixed with partial purified toxin was 4 mm. CD-Cholesterol was used in treatment as a drops which added to the affected eye and led to a decrease in corneal erosion area from 8 mm to 4 mm.

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Published

2023-02-08

Issue

Section

Biology

How to Cite

Effect of Some Materials on Productivity and Activity of -Hemolysin which Produced by Methicillin Ressistant Staphylococcus Aureus. (2023). Iraqi Journal of Science, 57(1B), 359-352. https://ijs.uobaghdad.edu.iq/index.php/eijs/article/view/7771

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