Association of CTLA-4 Single Nucleotide Polymorphisms with Autoimmune Hypothyroidism in Iraqi Patients

Authors

  • Alya H. Yassin Biotechnology Department, College of Science, University of Baghdad, Baghdad, Iraq https://orcid.org/0000-0002-6536-7132
  • Abdul-Kareem A. Al-Kazaz Biotechnology Department, College of Science, University of Baghdad, Baghdad, Iraq
  • Abbas M. Rahma National Diabetes Center, University of Mustansiriya, Baghdad, Iraq
  • Taha Y. Ibrahim Ibn Sina Research Center, Industrial research and development, Ministry of Industry, Baghdad, Iraq

DOI:

https://doi.org/10.24996/ijs.2022.63.7.13

Keywords:

CTLA-4, thyroid, susceptibility, autoantibody, case/control study

Abstract

Genetic and environmental factors are believed to have a key role in the development and pathogenesis of autoimmune thyroid diseases (AITD). This study aimed to investigate the association between two CTLA-4 gene single nucleotide polymorphisms (SNPs) CT60/rs3087243 and CT61/rs11571319 with autoimmune thyroiditis in a sample of Iraqi patients. Seventy-five patients (67 females, 8 males) and eighty-eight subjects (79 females and 9 males) matched in age, gender, and ethnicity as a control group. Thyroid autoantibodies were present in females more than in males with a total positivity of anti-TPO of 92% and anti-TG positivity of
57.3 %. Thyroid evaluation tests including T3, T4, and TSH were abnormal only in patients not receiving L-thyroxine treatment for autoimmune hypothyroidism, while patients on L-treatment and control subjects had normal results of these tests. CTLA-4 genotype frequencies were consistent with Hardy-Weinberg equilibrium (HWE) with no significant differences (p > 0.05) between the genotypes of patients and the control group. Analysis of CTLA-4 genotype and allele frequencies in patients and controls indicated the lack of significant differences among these frequencies except for allele G of rs3087243 which was associated significantly (P= 0.032) with the disease with OR of 1.62, while allele A can have a protective effect with OR of 0.66. Alleles of rs11571319 showed no significant  ifferences despite the decreased frequency of G allele (83.33vs.87.5 %) and increased frequency of A allele (16.66vs.12.5%) in patients compared to controls. In conclusion, G allele of rs3087243 can be considered a risk factor for autoimmune hypothyroidism while no
association was found regarding rs11571319 in the Iraqi population. 

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Published

2022-07-31

How to Cite

Yassin, A. H. ., Al-Kazaz, A.-K. A. ., Rahma, A. M. ., & Ibrahim, T. Y. . (2022). Association of CTLA-4 Single Nucleotide Polymorphisms with Autoimmune Hypothyroidism in Iraqi Patients. Iraqi Journal of Science, 63(7), 2891–2899. https://doi.org/10.24996/ijs.2022.63.7.13

Issue

Section

Biotechnology

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