Dysregulation of Micro RNA-155 as a Biomarker in Iraqi Patients with Prostate Cancer

  • Jinan MJ Al-saffar
Keywords: Prostate cancer, TNF-α, IL-1α, mir-155, ELISA, RT-PCR

Abstract

Chronic inflammation can induce proliferative events and posttranslational DNA modifications in prostate tissues through oxidative stress. The miR-155 expression is abnormally increased in tumors. Therefore, this study aims to explore the clinical significance of serum TNF-α and IL-1α as well as miR-155 expression in prostate cancer (PC) patients.

A total of 50 blood samples from patients diagnosed with prostate cancer were collected from the Oncology Department, Baghdad Teaching Hospital, along with samples from 50 healthy volunteers. Serum TNF-α and IL-1α levels in Iraqi males with prostate cancer were estimated by using enzyme-linked immunosorbent

assay (ELISA). The total RNA was extracted from all subjects and converted to cDNA using Stem-Loop technique and the expression was measured by real-time PCR (RT-PCR) using SYBR Green with specific primers. The results showed that serum TNF-α and IL-1α levels were significantly higher in the clinical stage of tumor node metastasis (TNM) as compared to those in the control (p<0.05). The miR- 155 level was significantly elevated in the patients  compared with the control (p<0.05), being positively correlated with advanced stages. It is concluded that circulating miR-155 could act as a promising marker for the diagnosis and prognosis of PC.

Published
2021-03-30
How to Cite
Al-saffar, J. M. (2021). Dysregulation of Micro RNA-155 as a Biomarker in Iraqi Patients with Prostate Cancer. Iraqi Journal of Science, 62(3), 838-844. https://doi.org/10.24996/ijs.2021.62.3.14
Section
Biotechnology