Molecular Detection of Human Herpes Virus-8 in Prostatic Adenocarcinoma and Benign Prostatic Hyperplasia Tissues by DNA -In Situ Hybridization

  • Zainab Mahdi Saleh Department of Biotechnology, Collage of Science, University of Baghdad, Baghdad, Iraq
  • Jinan Al-Saffar Department of Biotechnology, Collage of Science, University of Baghdad, Baghdad, Iraq
  • Saad Hassan Pathology Research Unit, College of Medicine, University of Baghdad, Baghdad, Iraq
Keywords: HHV-8, Prostatic Adenocarcinoma, Benign Prostatic-Hyperplasia, Chromogenic In Situ Hybridization

Abstract

Human Herpes Virus-8 (HHV-8) is a sexually transmitted viral infection that can infect the prostate epithelium in immunocompromised adults. Recently, HHV-8 was related to the development and progression of several human malignancies like prostatic adenocarcinoma. This retrospective research was designed to analyze the distribution and possible impact of HHV-8 infection on prostatic adenocarcinogenesis. A total number of one hundred formalin-fixed prostatic tissues were enrolled in this research; forty Prostate Adenocarcinoma (PAC) biopsies, forty biopsies from Benign Prostatic Hyperplasia (BPH), and twenty Apparently Normal Prostatic Tissues (ANPT) as a control group. Detection of HHV -8 DNA was achieved by a highly-sensitive variant of Chromogenic In Situ Hybridization (CISH) technique. In the mean age of PAC patients was 64.08±7.54years. Detection of CISH reactions for HHV 8- DNA was observed in tissues of 70% (28 out of 40) of PAC patients and 30% (12 out of 40) of BPH tissues, whereas no positive reactions were detected in the ANPT group. Detection of CISH reactions for HHV 8- DNA was observed in 55.6%  of tissues of PAC patients  with well grade histopathological examination,  87.5% of moderate grade, and 69.6% of poorly differentiated grade. It can be concluded that HHV-8 infection might contribute in prostate oncogenesis, together with other essential oncogenic viruses.

Published
2021-01-30
How to Cite
Saleh, Z. M., Al-Saffar, J., & Hassan, S. (2021). Molecular Detection of Human Herpes Virus-8 in Prostatic Adenocarcinoma and Benign Prostatic Hyperplasia Tissues by DNA -In Situ Hybridization. Iraqi Journal of Science, 62(1), 96-107. https://doi.org/10.24996/ijs.2021.62.1.9
Section
Biotechnology