The Synergistic Effects of Chitosan-Alginate Nanoparticles Loaded with Doxycycline Antibiotic Against Multidrug Resistant Proteus Mirabilis, Escherichia Coli and Enterococcus Faecalis

Authors

  • Wasan Nabeel Alsaady Department of Biotechnology, College of Science, University of Baghdad, Bagdad, Iraq
  • Israa Ali Zaidan Department of Biotechnology, College of Science, University of Baghdad, Bagdad, Iraq

DOI:

https://doi.org/10.24996/ijs.2020.61.12.6

Keywords:

chitosan-alginate, antibacterial, Enterobacteriaceae, synergistic effect, Enterococcus faecalis, Proteus mirabilis, doxycycline, Enterococcaceae

Abstract

     This study focused on the synthesis of chitosan-alginate (CH-ALg) nanoparticles by ionotropic gelation technique using sodium alginate and calcium chloride. The particle size of the synthesized nanoparticles was confirmed by atomic force microscope (AFM) and it was 61.9 nm. While the nature of functional groups present in chitosan nanoparticles was determined by FT-IR analysis. The antibacterial activity of chitosan-alginate was tested against multidrug resistance (MDR) gram- positive (Enterococcus faecalis) and gram-negative (Proteus mirabilis) bacteria. The results showed a significant effect against MDR isolates. The nanoparticles were loaded with the antibiotic doxycycline in order to improve the antibacterial activity and drug delivery efficiency. The synergistic effects of the biosynthesized chitosan-alginate and the loaded doxycycline at different concentrations against MDR bacteria were also investigated. The results showed that doxycycline-loaded nanoparticles have superior effectiveness compared to native doxycycline against gram negative and gram positive bacteria.

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Published

2020-12-29

Issue

Section

Biotechnology

How to Cite

The Synergistic Effects of Chitosan-Alginate Nanoparticles Loaded with Doxycycline Antibiotic Against Multidrug Resistant Proteus Mirabilis, Escherichia Coli and Enterococcus Faecalis. (2020). Iraqi Journal of Science, 61(12), 3187-3199. https://doi.org/10.24996/ijs.2020.61.12.6

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