Assessment of Genotoxicity of Citrobacter freundii Bacteriocin on Bone Marrow Cells in Albino Mice

Authors

  • Rasha Noori Hammad Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq
  • Hind Hussein Obaid Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq

DOI:

https://doi.org/10.24996/ijs.2020.61.5.8

Keywords:

Genotoxicity, Citrobacter Freundii, Bacteriocin, Bone Marrow Cells, Micronucleus(MN), Comet Assay

Abstract

Genotoxic effects of crude bacteriocin extracted from Citrobacter freundii were detected on albino mice bone marrow cells in vivo, using micronucleus (MN) and comet assay. The mice were administered intraperitoneally with 37.5, 75, 150 and 300 mg/kg of the extract for 24 hours. C. freundii was isolated from patients suffering from urinary tract infections (UTI). The bacteriocin producing isolates were determined using cup assayand the most efficient bacteriocin producers were selected. Bacteriocin was extracted from the efficient isolates via the induction with Mitomycin-C (2 mg/ml). Bacteriocin activity (320 U/ml) was determined by well diffusion method, while the protein concentration (2900µg/ml) was estimated by Lowery method. The results showed an acute dose-dependent toxic effect of the crude bacteriocin ; The higher doses (150 and 300 mg/kg) caused a significant increase (P≤0.05) in the micronuclei frequency in the bone marrow cells (4.62 and 5.37%, respectively (. Furthermore, DNA damage   increased significantly (P≤0.05) and proportionally to higher bacteriocin doses (75, 150 and 300 mg/kg), as demonstrated by increased values of  tail length  (145.18, 267.73 and 295.08 %,( %DNA in tail (8.05, 13.87 and 14.31 %(, and olive tail moment (13.25, 22.72 and 25.85 % , respectively.

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Published

2020-05-28

Issue

Section

Biology

How to Cite

Assessment of Genotoxicity of Citrobacter freundii Bacteriocin on Bone Marrow Cells in Albino Mice. (2020). Iraqi Journal of Science, 61(5), 999-1007. https://doi.org/10.24996/ijs.2020.61.5.8
Crossref
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Katarina G. Marković, Mirjana Ž. Grujović, Maja G. Koraćević, Danijela D. Nikodijević, Milena G. Milutinović, Teresa Semedo-Lemsaddek, Milan D. Djilas (2022)
Colicins and Microcins Produced by Enterobacteriaceae: Characterization, Mode of Action, and Putative Applications. International Journal of Environmental Research and Public Health, 19(18), 11825.
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Mahmood A.E. (2021-10-26)
Determination of some virulence factors of Citrobacter freundii isolated from Iraqi patients. Iraqi Journal of Science, 62(9), 3358-3365.

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