EXTRACTION, PURIFICATION AND CHARACTERIZATION OF CYTOSINE DEAMINASE FROM Saccharomyces cerevisiae

Authors

  • Mohammed Nader * Genetic Engineering and Biotechnology، Institute for Post graduate Studies، Baghdad - Iraq.
  • Safaa Al-Maeny * Genetic Engineering and Biotechnology، Institute for Post graduate Studies، Baghdad - Iraq.

DOI:

https://doi.org/10.24996/ijs.2010.51.4.%25g

Keywords:

EXTRACTION, CHARACTERIZATION

Abstract

The cytosine deaminase was extracted by using organic solvent toluene and the value of specific activity of crude extract was (9.6) mu/mg protein . The enzyme was purified in few steps including precipitating the enzyme by using ammonium sulfate at defferent saturation percent, The optimum percent was 60% saturation percent which gave value of specfic activity of (12.5) mu/mg protein fold of purification was (1.302) times and the yield was 4.82% . Then the enzume solution was passed through DEAE – cellulose column chromatography. The peaks was collected and the value of specific activity was measured which was (358) mu/mg protein, fold of purification was (37.29) times, and the yield was (2.55)%.
The enzyme solution was concentrated and passed through sephadex G-200 gelfilteration and the value of specific activity was (400) mu/mg protein, fold purification (41.66) times and yeid was 1.19%.

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Published

2024-09-27

Issue

Section

Biotechnology

How to Cite

EXTRACTION, PURIFICATION AND CHARACTERIZATION OF CYTOSINE DEAMINASE FROM Saccharomyces cerevisiae. (2024). Iraqi Journal of Science, 51(4), 598-606. https://doi.org/10.24996/ijs.2010.51.4.%g

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