IMMUNONOLOGICAL EVALUATION AND ACUTETOXICITY STUDY WITH FERTILITY EXAMINATION FORTHE EFFECT OF AQUEOUS EXTRACT FROM DRIEDFRUITS OF Piper nigrum L. IN MICE

Abstract

The research work was conducted to investigate the effect of oral administration
of water extract of black pepper at doses of (1, 5) mg/kg body weight for two weeks
daily by determining the genotoxic effect (mitotic index), evaluation of
immunological effect (IgG, IgM, IgA, C3, C4) and measuring fertility hormones
(follicles stimulation hormone/FSH, lutenising hormone/LH) levels with
histopathological examinations of female albino swiss mice ovaries in comparison
with control (normal saline). A clear effect in increasing mitotic activity was reveled
for both doses in comparison with control. Results also showed a significant
increase in the value of the all immunological parameters at both doses in
comparison with control. Also obvious raise was seen in the level of FSH and LH
for both doses when compared with normal saline treated mice with no significant
damage seen in female ovaries tissue, in fact there were certain clinicopathological
changes in mice ovaries tissue which were represented by increasing in the numbers
of primary and secondary follicles and in the numbers of corpus luteum at both
doses.