EXTRACTION AND PURIFICATION OF β-galactosidaseFROM NEW BORN SHEEP BRAIN.

Authors

  • Ali Abed Department of Science, College of Basic Science, University of Al-Mustansryia. Baghdad- Iraq.

DOI:

https://doi.org/10.24996/ijs.2010.51.3.%25g

Keywords:

PURIFICATION, BORN

Abstract

β -galactosidase enzyme was extracted from the new born sheep brain by nine
solutions, It was found that the0.2 M sodium acetate and 0.2M NaCl give a highest
specific activity of crude enzyme. This enzyme was concentrated by four methods,
includes: concentration by ammonium sulfate (20-60%); old acetone; ethanol (30%-
60%) and by ultra filtration. It was found that the cold acetone was the better. The
purification folds were about 124.78 times and yield about 77.39%. The molecular
weight of this enzyme about 185.942 Kilo Dalton.

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Published

2024-09-27

Issue

Section

Biology

How to Cite

EXTRACTION AND PURIFICATION OF β-galactosidaseFROM NEW BORN SHEEP BRAIN. (2024). Iraqi Journal of Science, 51(3), 437-443. https://doi.org/10.24996/ijs.2010.51.3.%g

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