EVALUATION OF IMMUNE RESPONSE IN PATIENTS WITH CHRONICLYMPHOCYTIC LEUKEMIA. II: HUMORAL IMMUNE RESPONSE

Muhammed   Al-Dabagh; Kasim   Al-Mayah

doi:10.24996/ijs.2011.52.3.%g

Authors

Muhammed Al-Dabagh Unit of Medical Research, College of Medicine, University of Al-Nahrain. Baghdad – Iraq
Kasim Al-Mayah Unit of Medical Research, College of Medicine, University of Al-Nahrain. Baghdad – Iraq

DOI:

https://doi.org/10.24996/ijs.2011.52.3.%25g

Keywords:

IMMUNE, RESPONSE

Abstract

Chronic lymphocytic leukemia (CLL) is a hematologic malignancy characterized by
progressive accumulation of lymphocytes. This study aimed to evaluate humoral
immunity in patients with CLL via estimation of serum levels of immunoglobulins IgG,
IgA, and IgM and two cytokines: interleukin-4 (IL-4) and interleukin-10 (IL-10). Blood
samples were collected from 48 patients with CLL (28 treated and 20 untreated). Other
20 blood samples were collected from healthy individuals as control group. Single radial
immune-diffusion assay was used to estimate the serum levels of immunoglobulins.
Enzyme linked immunosorbent assay (ELISA) was used to estimate serum concentration
of IL-4 and IL-10. The study showed unsignificant increase in serum concentration IgG
and IL-10 while there was unsignificant decrease in IgA, IgM, and IL-4 in treated and
untreated groups as compared with the healthy control group. These data indicates a
little or unimportant effects of IL-4 and IL-10 on immunoglobulin production in studied
patients.