THE USAGE OF ß-GLUCAN WHICH EXTRACTED FROMSaccharomyces cerevisiae YEAST CELLS WALL ASIMMUNOMODULATOR

Abstract

Carbohydrates (polysaccharides ) were extracted from Saccharomyces cerevisiae
cell wall by hot alkaline solution, the polysaccharides concentration was 96 mg /ml ,
while protein concentration was 500 μg /ml ,then primary purification method was
done to separated carbohydrates from other protein materials, at this time ,
carbohydrates concentration was 250 mg /ml, while protein concentration was 148
μg /ml .Then partial purification using Ion exchange technique was done, and the
results revealed ,four peaks were separated ,every peak was collected ,dialysed , and
measured the carbohydrates concentration which were 300, 50, 33.4 and 32 mg/ml
respectively, while Lawery method did not detect any protein materials in samples.
The four peaks were examined as immunomodulator through the animal (mice) that
were divided into 4 groups which depend upon the peaks series, were injected
intraperitoneal and orally administration with constant concentration 500 μg /ml
twice, then read the blood picture for immune cells, the second group was reported a
significant increasing in all cells which are (2.968 ± 0.316 ), (1.56± 0.3), (0.546 ±
0.291) Lymphocyte, Granulocytes and monocyte respectively while the cell
percentage for first, third, and fourth peaks were for lymphocyte (2.292 ± 0.3) (1.83
±0.32) and (2.67 ± 0.5) respectively, for Granulocytes (0.83± 0.23) (0.53 ± 0.22)
and (0.6 ± 0.35) respectively, while for Monocytes (0.3 ± 0.19) (0.03 ± 0.01) and
(0.3± 0.02) respectively when compared with control group. Organs crossly
examination was showed hypertrophy of liver and spleen, while microscopically,
there were infiltration of lymphocytes around the blood vessels in liver, lung, and
peritonea, and in white pulp of spleen.