BATCH AND FLOW-INJECTION SPECTROPHOTOMETRIC DETERMINATION OF SODIUM CEFOTAXIME IN PHARMACEUTICAL PREPARATIONS

Mouyed Al-Abachi; Hind  Al-Ward; Yassmin Mohammad

doi:10.24996/

Authors

Mouyed Al-Abachi Department of chemistry, College of Science, University of Baghdad. Baghdad – Iraq.
Hind Al-Ward Department of chemistry, College of Science, University of Baghdad. Baghdad – Iraq.
Yassmin Mohammad Department of chemistry, College of Science, University of Baghdad. Baghdad – Iraq.

DOI:

https://doi.org/10.24996/

Keywords:

Sodium Cefotaxime, Spectrophotometric determination, Thymol, Diazotization and coupling, Flow injection

Abstract

Simple and sensitive batch and Flow-injecton spectrophotometric methods (FIA)
for the determination of Sodium Cefotaxime in pure form and in injections were
proposed. These methods were based on a diazotization and coupling reaction
between diazotized Sodium Cefotaxime and thymol in alkaline medium to form an
intense red water-soluble dye that is stable and has a maximum absorption at 522
nm. A graphs of absorbance versus concentration show that Beer’s law is obeyed
over the concentration range of 8-160 and 50-2500 μg.ml-1 of Sodium Cefotaxime,
with detection limits of 1.56 and 1.32 μg.ml-1 of Sodium Cefotaxime for batch and
FIA methods respectively. The FIA procedure sample throughput was 65 h-1. All
different chemical and physical experimental parameters affecting on the
development and stability of the colored product studied and the proposed methods
were applied successfully on the determination of Sodium Cefotaxime injections
preparations.