Inhibitory Effects of Iron Oxide Nanoparticles Biosynthesized by Prodigiosin Pigment Against Clinical Bacteria Isolates of Pseudomonas aeruginosa

Abstract

The current study aimed to reveal the biosynthesis technique of Fe₂O₃ nanoparticles by using prodigiosin pigment production from Serratia marcescens. One hundred and twenty samples were obtained from burn and wound infections to isolate Pseudomonas aeruginosa. In addition, the optimum conditions for Fe₂O₃ nanoparticles biosynthesis were characterized via different methods, including ultraviolet-visible (UV-Vis), atomic force microscope (AFM), fourier transforms infrared (FT-IR) spectroscopy analysis, X-ray diffraction (XRD), and field emission scanning electron microscopy (FE-SEM). In particular, spherical shape particles were observed utilizing FE-SEM technique. In addition, the analysis of AFM revealed Fe₂O₃ NPs with an average diameter size of 50.73 nm.  The influences of varied concentrations of iron nanoparticles (6.25, 12.5, 25 , 50, 100and 200 mg/ml) on a bacterial isolate of Pseudomonas aeruginosa were examined. The maximum inhibition zone of P. aeruginosa was 24 mm at a concentration of 200 mg/ml of Fe₂O₃ NPs, whereas the minimum inhibition zone were 10 mm located at 25 mg/ml Fe₂O₃ NPs concentrations.