Titanium Dioxide Nanoparticles: A Novel Approach for Inhibiting Human Papillomavirus

Authors

  • Sharafaldin Al-Musawi College of Food Sciences, Al-Qasim Green University, Babylon, Iraq https://orcid.org/0000-0001-9414-9663
  • Abbas. K. Almansorri Faculty of Biotechnology, Al-Qasim Green University, Babylon, Iraq
  • Hider. M. H. Al-Shirifi Dept. of Environmental Health, College of Environmental Sciences, Al-Qasim Green University, Babylon, Iraq
  • Bara B Ahmed Faculty of Biotechnology, Al-Qasim Green University, Babylon, Iraq
  • Adawiya J. Haider Laser Science and Technology Branch, Department of Applied Sciences, University of Technology, Baghdad, Iraq https://orcid.org/0000-0002-2191-0500

DOI:

https://doi.org/10.24996/ijs.2024.65.3.13

Keywords:

Papillomavirus, Titanium oxide, nanoparticles, Antiviral Activity, drug-resistant

Abstract

     Nanotechnology products such as titanium dioxide nanoparticles (TiO2-NPs) can be used for viral infections because of their unique characteristics. The current study aimed to determine the impact of TiO2-NPs on HPV type 1 and 2 infections. The characterization of these NPs was performed using dynamic light scattering (DLS), field emission scanning electron microscopy (FESEM), high-resolution transmission electron microscopy (HRTEM), and X-ray diffraction (XRD). The MTT assay was used to determine the toxic impacts of TiO2-NPs on BHK-21 cells. The efficiency of TiO2-NPs was performed using several parameters, including TCID50 and RT-PCR assays. An indirect immunofluorescence assay (IFA) was performed to estimate the inhibitory impact of TiO2-NPs on viral antigen expression, and Acyclovir was used as a reference medicine. When the human papilloma type 1 and 2 viruses exposed to TiO2-NPs at high doses (100 μg/mL) produced 0.3, 1.1, 2.3, and 3.3 log10 TCID50 decreases in infective virus load when compared with control viruses (P<0.0001), these TiO2-NPs doses were related to 24.9%, 35.1%, 47.2%, 59.5%, and 66.6% inhibition percentages that were determined depending on the viral titer as compared to virus control. It is concluded that TiO2-NPs have strong potential for the treatment of face and labial lesions caused by papillomaviruses 1 and 2 and could be used in topical formulations.

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Published

2024-03-29

Issue

Section

Biotechnology

How to Cite

Titanium Dioxide Nanoparticles: A Novel Approach for Inhibiting Human Papillomavirus. (2024). Iraqi Journal of Science, 65(3), 1320-1330. https://doi.org/10.24996/ijs.2024.65.3.13

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