Study of the Effect of Raphanus Sativus, Urtica dioica and Curcuma longa Plant Extracts for Pollution Treatment by Fish's Provender

Abstract

This study was conducted to estimate the activity of crude extracts of the horseradish and nettles, turmeric plant and a combination of these combined plants extracts for the treatment of heavy metal pollution like zinc and cobalt that accumulate in fish by adding them to fish provender. This experiment was carried out during the September, October, November months, by using 40 gold fish that distributed in five basins, the heavy metals ratio (zinc and cobalt) were determined in the bodies of fish before the pollution in each basin. It has been added salts of zinc and cobalt to the basins in order to getting the concentration ratio 10 mg/L for each of the metals in the basins water, after 14 days have been re-determining of the concentration of metals in the basins and before the treatment. Attended three crude plants extract by using alcohol and water, as well as detection of the active compounds that may exist in plant extracts That showed the horseradish extract contains alkaloids , terpenes , saponins , flavonoids, resins , glycosides , coumarins and tannins, while the nettles extract and turmeric extract contains the alkaloids , flavonoids , saponins, resins, tannins and glycosides. also, the toxicity test was carried out for determine the toxicity of extracts, which proved that the crude extracts were non-toxic, then, from the crude extracts were prepared a different concentrations with the singly form and blended to remove a pollution from the bodies of the fish in the four basins, while, The fifth basin was used as a control basin that a provender was added to it only. After 14 days, the concentration of zinc and cobalt were determined in bodies of fish, the concentration of zinc was ranging from 1.7810 mg/L to 2.4527 mg/L, while the cobalt concentration was ranging from 0.0582 mg/L to 1.436 mg/L. The treatment was continued for 21 days, after that, heavy metal salts were determined in the bodies of fish. The concentration of zinc was from 0 mg/L to 2.0950 mg/L and cobalt was 0 mg/L. These results showed the existence of significant differences among the heavy metals concentrations in bodies of fish for different basins after 21 days of treatment. As it is shown by the results that the nettle extract was more efficient in the removal of zinc from the fish tissue. While, the horseradish extract was more efficient in the removal of cobalt from the fish tissue.